GC-MS Analysis of Strobilanthes crispus Plants and Callus

Abstract

Strobilanthes crispus or locally known as “bayam karang”, “pecah kaca”,
\n“jin batu” and “pecah beling” in Malaysia, has been traditionally used to
\nincrease immune system, treating kidney stones, treatment of diabetes
\nmellitus, treatment of high blood pressure and treatment of wound. Studies
\nexamining the phytochemical constituents reported that the leaves of this
\nplant contain ester glycosidic compound of caffeic acid, -voumaric acid, ,
\nvanilic acid, ferulic acid, syringic acids, sitosterol, campesterol,
\nhexadecanoic acid, methylester, lupeol, phytol, stigmasterol, flavonoid
\ncompounds such as (+)-catechin, (-)-epicatechin, rutin, and etc. While most
\nof the literatures focused on the chemical compounds present in the leaves
\nof S. crispus, none have been reported for the phytochemical constituents of
\nthe whole S. crispus plant including the leaf, stem, root or flower part.
\nBesides, there is also lacking report on the tissue culture generated from this
\nplant too. Thus, this study was carried out to profile the leaves, stems and
\nroots and callus cultures of S. crispus using gas chromatography mass
\nspectrometry (GC-MS) approach. Results revealed that this plant is rich with
\nsqualene, phytosterols such as stigmasterols and derivatives, sito-sterol,
\ncampesterols, as well as triterpenoids such as lupeol, amyrin and betulin.