Influence of Date Syrup as a Carbon Source on Bacterial Cellulose Production by <i>Acetobacter xylinum</i> 0416

Abstract

Abstract To improve bacterial cellulose ( BC ) applications, researchers have expanded their efforts with BC productivity and its properties. The objectives of this study are to evaluate the possibility and efficacy of using extracted date syrup as a carbon source for Acetobacter xylinum 0416 in BC production in a static culture. The sugar content of extracted date syrup was identified by HPLC . Different concentrations of dates and different culturing times were tested. The produced BC s were characterized and their respective properties were compared with a BC produced from Hestrin and Schramm ( HS ) medium. The most effective BC amount was attained with 3% (w/v) date in the medium on day 8. The modified HS medium ( DHS ) increased production by 68% ( P &lt; 0.05). Morphological analysis by scanning electron microscopy revealed more disorganized arrangements in BC fiber obtained from DHS medium ( BC ‐ DHS ) than that from HS medium ( BC ‐ HS ). Thermogravimetric analysis revealed no significant changes in BC ‐ DHS thermostability compared to BC ‐ HS . However, the crystallinity of BC ‐ DHS improved ( P &lt; 0.05). The results of Fourier transform infrared spectroscopy demonstrated that the carbonyl group in BC ‐ DHS is more active than that of BC ‐ HS . Furthermore, the properties of BC ‐ DHS showed excellent potential for use in many applications.