Tracking inter-institutional spread of NDM and identification of a novel NDM-positive plasmid, pSg1-NDM, using next-generation sequencing approaches

Abstract

A combined sequencing-by-synthesis and SMRTS approach can determine effectively the transmission clusters of bla_NDM and genetically characterize novel plasmids. Plasmid molecular epidemiology is important to understanding NDM spread as bla_NDM-positive plasmids can conjugate extensively across species and STs.