Construction of Bacillus subtilis strain engineered for expression of porcine β-defensin-2/cecropin P1 fusion antimicrobial peptides and its growth-promoting effect and antimicrobial activity

Abstract

The generated <i>B. subtilis</i> strain can efficiently express pBD-2/CP1 fusion antimicrobial peptide, the recovered pBD-2 and CP1 peptides possess potent antimicrobial activities to a variety of bacterial species <i>in vitro</i>. Supplementation of the engineered <i>B. subtilis</i> in pig feed obviously promote piglet growth and resistance to the colibacillosis.