Irreversible binding of carbon tetrachloride to microsomal phospholipids. Free radical nature of the reactive specie and alterations in the physico-chemical properties of the target fatty acids.

Abstract

Thin layer and gas liquid chromatographic analysis of the fatty acid methyl esters derived from liver microsomal phosphatidylcholine from animals treated with 14CC1-4 revealed a very similar composition to that observed during the analysis of the reaction products arised by the in vitro benzoyl peroxide initiated CC1-4 chemical addition to double bonds of fatty acid methyl esters or to fatty acids in phospholipids later transformed to methyl esters. The well known CC1-3 free radical nature of this in vitro chemical reaction gives support to the possibility for a CC1-3 free radical formation in vivo.