Ferric reducing anti-oxidant power assay in plant extract

Abstract

<p>The ferric reducing anti-oxidant power (FRAP) assay involved the following steps: a) preparation of samples, b) reactions and c) finally measuring absorbance of sample and standard at 700 nm using spectrophotometer. The samples were methanolic extract, different fractions (<em>n</em>-hexane, chloroform) and standard ascorbic acid. The solutions prepared were buffer solution (pH 6.6), 1% potassium ferricyanide solution, 10% trichloroacetic acid and 0.1% ferric chloride. </p><p><strong>Video Clip</strong></p><a href="https://www.youtube.com/v/s7BpxNbN5dE">Ferric reducing anti-oxidant power assay:</a>21 min 18 sec<p> </p>