Publication | Open Access
Simultaneous Determination of 20 -Agonists in Pig Muscle and Liver by High-Performance Liquid Chromatography/Tandem Mass Spectrometry
19
Citations
0
References
2011
Year
NutritionIllegal Residual Beta-agonistsMeat QualityLaboratory Animal StudyBody CompositionBioanalysisFeed AdditivePork TissuesAnalytical ChemistrySimultaneous DeterminationLiquid ChromatographyChromatographyHealth SciencesAnimal PhysiologyBiochemistryLiver PhysiologyPharmacologyDecision CapacityPhysiologyMass SpectrometryMetabolismMedicinePig MusclePharmacokineticsDrug Analysis
A method was developed to determine 20 illegal residual beta-agonists in pork tissues, including muscle and liver simultaneously. The samples were hydrolyzed by beta-glucuronidase, purified by PCX SPE cartridges, and detected by HPLC coupled with electrospray ionization MS/MS operating in the positive ion mode. Matrix-fortified calibration was performed to compensate for the matrix effect and loss in sample preparation. Decision limit ranged from 0.05 to 0.23 microg/kg in muscle and 0.05 to 0.57 microg/kg in liver. Decision capacity ranged from 0.11 to 0.4 microg/kg in muscle and 0.16 to 0.79 microg/kg in liver. In Food Analysis Performance Assessment Scheme proficiency test 0287, a pig liver test material containing 13 beta-agonists was analyzed using the method developed, and clenbuterol and ractopamine were confirmed as being present. Z-scores for clenbuterol and ractopamine were 0.2 and 0.6, respectively.