World Journal of Gastroenterology · 2003 · 44 citations · 47 references
Life SciencesOncologyPharmacologyApoptosisCancer PharmacologyCell SignalingApoptosis In CancerApoptosis ModulationApoptosis RegulationApoptotic SignalingBcl-2 Family ProteinsGastric CancerBcl-2 InhibitionBcl-2 Protein ExpressionBcl-2 Protein RegulationCell Growth RegulationCell Proliferation IndicesPeroxisome Proliferator Receptor SignalingPeroxisome Proliferator-activated Receptor Gamma AgonismWestern Blotting
AIM: To investigate the influence of peroxisome proliferator-activated receptor gamma (PPARgamma) ligand, 15-deoxy-12, 14-prostaglandin J2 (15dPGJ2) on the proliferation and apoptosis of MCG-803 human gastric cancer cell lines. METHODS: Cell proliferation was measured by 3H-TdR assay. Apoptosis was determined by ELISA and TUNEL staining. Protein and mRNA level of bcl-2 family and COXs were measured by Western blotting and Northern blotting respectively. PGE2 production was examined by RIA. RESULTS: 15dPGJ2 inhibited cell growth and induced apoptosis of MCG-803 cells. The COX-2 and bcl-2/bax ratios were decreased following 15dPGJ2 treatment. The PGE2 production in supernatants was also decreased. These changes were in a dose-dependent manner. CONCLUSION: 15dPGJ2 may be a useful therapeutic agent for the treatment of gastric cancer.
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