Pharmacokinetics and Absolute Bioavailability of Breviscapine in Beagle Dogs

Abstract

Scutellarin in plasma was determined by RP HPLC with ultraviolet detection at 335 nm. According to a randomized two period crossover design, the beagle dogs were iv 90 mg and oral admini strated of 1800 mg of scutellarin, respectively, with a 6 day washout. The limit of quantification for scutellarin was 0.025 μg/ml, the intra and inter day RSD s were less than 8.31%, and the average reco very was above 93.2%. It was found that scutellarin in plasma was unstable under room temperature. The pharmacokinetics behavior of scutellarin iv could be described by a three compartment model. The absolute bioavailability of scutellarin for oral administration was (0.40±0.19)%. The elimination of iv breviscapine in dogs was fast, but it was almost not absorbed for oral administration.