Isolation of Salmonella Typhimurium from poultry eggs and meat of Tarai region of Uttaranchal

Abstract

Presence of Salmonella in samples, 100 each of chicken eggs and meat, collected from various retail outlets of the Tarai region of Uttaranchal, was detected by polymerase chain reaction (PCR) using InvA gene primer, a rapid and sensitive DNA based method. The results were further validated by established conventional method of Salmonella isolation and biochemical tests, including slide agglutination test (poly “O” antiserum specific for Salmonella ). Of 200 samples analyzed, 4 (one meat and 3 egg) were found to be contaminated with Salmonella enterica var Typhimurium. Further, the antibiogram of the isolates revealed sensitivity in the decreasing order to Chloromphenicol, Colistin, Polymixin, Enrofloxacin, and Ciprofloxacin.