Glutathione Content and Glutathione to Hydroxycinnamic Acid Ratio in Vitis vinifera Grapes and Musts

Abstract

Glutathione (GSH) was analyzed by high performance liquid chromatography following derivatization using Ellmann9s reagent in the berries of 28 grape varieties from different locations and in the corresponding white musts. The hydroxycinnamic acid to glutathione molar ratios were determined for each sample. GSH concentration varied from 56 to 372 µmole/kg in grapes and from 46 to 333 mM in musts. The hydroxycinnamic acids to glutathione ratio was consistently higher in the entire berries than in the corresponding musts. The extreme values were 1.3 and 12.7 in berries and 0.6 and 10.5 in musts. Growing site related differences were also pointed out.