Science and Technology of Advanced Materials · 2016 · 27 citations · 37 references
Natural SciencesBiochemistryChemistryAnalytical ChemistryBiosensorsSpectroscopyChemical SensorsFluorescence SpectroscopyLuminescent MaterialsOptical SpectroscopyPhotochemistrySpectroscopy Of BiomoleculesLinear RangeLuminescence-based AssaysLuminescent BiosensorsLuminescent SensorsMetal-ligand Charge TransferMethod Detection LimitPhotoactive Metal ComplexesSelectivity
In this study, we report the use of a cyclometalated luminescent iridium(III) complex for the visualization of thiols. The detection of glutathione (GSH) by complex 1 is achieved through the reduction of its phendione N^N donor, which influences the metal-to-ligand charge-transfer (MLCT) of the complex. Complex 1 produced a maximum threefold luminescence enhancement at 587 nm in response to GSH. The linear detection range of 1 for GSH is between 0.2 and 2 M equivalents of GSH, with a detection limit of 1.67 μM. Complex 1 also displays good selectivity for thiols over other amino acids.
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