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Some New Bifunctional Reagents for the Conjugation of a Protein with an Amino Compound in Water
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1975
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Protein ChemistryNew Bifunctional ReagentsBioorganic ChemistryProtein AssemblyBiochemistryProtein FoldingNatural SciencesBioconjugationMolecular BiologyReagent IiiPeptide SynthesisProtein EngineeringAmino CompoundChemistryReagentSymmetrical Bifunctional ReagentsTryptophan ResiduesProtein Purification
Five symmetrical bifunctional reagents (I-V) have been synthesized and tested for their usefulness for the conjugation of albumin with tryptophan, in aqueous solution. With I and II about 17, with IV and V about 8 tryptophan residues could be introduced in the native protein. The difference is discussed. Reagent III has a very low solubility in water but may be useful if organic solvents are permissible.