Studies with Cells From Marine Fish in Tissue Culture.

Abstract

Cells of tissues obtained from marine fish were shown to grow by the explant method at 20 °C in a medium designed for mammalian cells. Preparation of monolayers from cells dispersed by primary trypsinization was accomplished when additional sodium chloride was added to mammalian cell media. Cells in continuous culture were found to multiply more efficiently (as regards time required for sheet formation and number of cells required for satisfactory growth) than did cells in primary culture.