Detection of Renibacterium salmoninarum in tissue sections by different methods - A comparative study with special regard to the indirect immunohistochemical peroxidase technique.

Abstract

Various staining methods (the Gram and periodic acid stains;the indirect fluorescence antibody technique and the indirect peroxidase procedure) were compared for their ability to detect the kidney disease bacterium (Renibacterium salmoninarum) in the tissues of rainbow trout (Salmo gairdneri) fixed by various methods (fresh frozen tissue, frozen formalin-fixed tissue, formalin-or Bouin's-fixed paraffin-embedded tissue). The results demonstrated that only the indirect peroxidase technique gave positive results regardless of the fixation method used to prepare the tissue. The other methods were either less sensitive or yielded negative results after some tissue fixation methods.