Biochemistry · 2014 · 63 citations · 34 references
Life SciencesBiochemistryMolecular BiologyBiosensorsCell SignalingMolecular ImagingChemical Probe DiscoveryDisulfide Bond FormationFluorescence MicroscopyMolecular Probe DevelopmentPhotophysics Of Fluorescent ProbesSulfur ChemistryAnalytical Methods DevelopmentChromophore FormationFluorescence ProbesFluorescent Probe DesignFluorescent ProbesLive-cell Imaging ProbesMolecular Imaging ProbesSensitivity
The discovery of hydrogen sulfide (H2S) as a novel gasotransmitter for cell signaling and other pathophysiological processes has spurred tremendous interest in developing analytical methods for its detection in biological systems. Herein, we report the development of a highly responsive and selective genetically encoded H2S probe, hsGFP, for the detection of H2S both in vitro and in living mammalian cells. hsGFP bestows a combination of favorable properties, including large fluorescence responses, high efficiency in folding and chromophore formation, and excellent sensitivity and selectivity toward H2S. As a genetically encoded probe, hsGFP can be readily and precisely localized to subcellular domains such as mitochondria, cell nuclei, and ion channels. hsGFP was further utilized to image H2S enzymatically produced from l-cysteine in human embryonic kidney (HEK) 293T cells.
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Engineering and characterization of a superfolder green fluorescent protein
J.D. Pédelacq, Stéphanie Cabantous, Timothy H. Tran et al. · Nature Biotechnology · 2005 · 2.5K citations
Guangdong Yang, Lingyun Wu, Bo Jiang et al. · Science · 2008 · 2.3K citations · Full text