A Rapid Method for Determination of the Acid-soluble Nucleotides in Fish Muscle by Concave Gradient Elution

Abstract

By introducing a concave gradient elution system with increasing sodium chloride concentration and decreasing pH, the column chromatographic method for determination of nucleotides and related compounds in fish muscle was simplified. HXR+HX, IMP, AMP, ADP and ATP can be separated completely from each other within 3 hrs and the recoverÍes are satÍsfactory. The dÍstrÍbution pattern of these compounds in the muscles of yellow tail, Pacific cod and fan shell was examined by the improved method.