Anti-Inflammatory and Acetylcholinesterase Inhibition Activities of Globularia Alypum

Abstract

 Abstract—The Globularia alypum methanolic extract (GAME) was evaluated for the anticholinesterase, anti 5lipoxygenase, NO production inhibitory activities and the transcriptional regulation pathway. Interestingly, GAME showed an important anti-inflammatory activity in a dose dependant manner, and inhibited nitric oxide (NO) production via transcriptional regulation of iNOS gene by (66%) at 150mg/L in IFN-γ/LPS stimulated RAW 264.7 macrophages. In addition, both Globularia alypum showed a 5-Lipoxygenase inhibitory activity with IC50 value of 79±0.8mg/L. Acetyl-cholinesterase inhibition was assessed by modification of the Ellman’s method. Globularia alypum exhibited a strong activity against cholinesterase with IC50 value 9.33±0.47mg/L. The data suggest that Globularia alypum extract could be used as a natural inhibitor of oxidation and alzheimer disease, and since GAME induced a potent anti-inflammatory suggest its potent use for the treatment of inflammatory diseases. Index Terms—Globularia alypum, anti-inflammatory activity, anti- cholinesterase.