Studies on Nuclear and Mitochondrial DNA of Saccharomyces cerevisiae

Abstract

A common feature of eukaryotic organisms is the localization of DNA in at least two sites, the nucleus and the mitochondrion. Our studies on the yeast Saccharomyces cerevisiae have been concerned with both species of DNA. We have examined the size and structure of the nuclear DNA of this eukaryote and have found evidence for uninemy. Our data can be reasonably interpreted that the chromosomes each contain a single, DNA duplex (Blamire et al., 1972a; Blamire et al., 1972b). The yeast nuclear DNA molecules were assumed to be linear, since no evidence was obtained for circularity. The chromosomal localization of the 18 S and 28 S ribosomal RNA cistrons has also been investigated, and approximately 70 % of these cistrons have been assigned to chromosome 1 in S. cerevisiae (Finkelstein et al., 1972). This report describes these investigations as well as some new information regarding chromosome structure and the localization...