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A Simple Analysis of Purine and Pyrimidine Nucleotides in Plant Cells by High-Performance Liquid Chromatography
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1987
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Plant PhysiologyEngineeringBotanyPlant Phenolic CompoundsMolecular BiologyBiosynthesisBioanalysisPlant CellsPyrimidine NucleotidesPhytochemicalPlant CytologyChromatographyBiochemistryAbstract PurineHigh-performance Liquid ChromatographyPlant MetabolismNatural SciencesBiotechnologyPlant Cell CulturePhytochemistryPlant Biochemistry
Abstract Purine and pyrimidine nucleotides, extracted from cultured plant cells with 6 % perchloric acid, were separated directly with HPLC using anion-exchange Shimpack WAX -1 column. More than fifteen nucleoside mono-, di-, and triphosphates and nucleotide sugars were clearly separated and quantified without any interference from plant phenolic compounds.