pNEB193-derived suicide plasmids for gene deletion and protein expression in the methane-producing archaeon, Methanosarcina acetivorans

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

Steffan N. Ho, Henry D. Hunt, Robert M. Horton et al. · Gene · 1989 · 7.5K citations

Natural Sciences, Genetics, Directed Evolution +6

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

Robert M. Horton, Henry D. Hunt, Steffan N. Ho et al. · Gene · 1989 · 3.1K citations

Engineering, Hybridisation, Hybridization +13

High efficiency transformation of E.coli by high voltage electroporation

William J. Dower, Jeff F. Miller, Clifton W. Ragsdale · Nucleic Acids Research · 1988 · 2.8K citations · Full text

E. Coli, Engineering, Molecular Biology +17

A Third Bacterial System for the Assembly of Iron-Sulfur Clusters with Homologs in Archaea and Plastids

Yasuhiro Takahashi, Umechiyo Tokumoto · Journal of Biological Chemistry · 2002 · 446 citations · Full text

A conspicuous nickel protein in microbial mats that oxidize methane anaerobically

Martin Krüger, Anke Meyerdierks, Frank Oliver Glöckner et al. · Nature · 2003 · 376 citations

Engineering, Biogas, Conspicuous Nickel Protein +7