RETENTION CHARACTERISTICS OF VARIOUS PROTEINS IN FLOW FIELD-FLOW FRACTIONATION : EFFECTS OF PH, IONIC STRENGTH, AND DENATURATION

Abstract

Department of Chemistry, Sejong University, Seoul 133-747, KoreaReceived March 24, 1999The study investigated the effect of carrier composition (ionic strength and pH) on the retention of various pro-teins in flow field-flow fractionation (Flow FFF) as well as the conformational change of Bovine Serum Albu-min (BSA) with urea concentration, storage time and temperature. The study found that the retention of proteinin Flow FFF increased with the ionic strength of the carrier liquid. Most proteins were well solubilized at pH =7-8. The hydrodynamic diameters obtained from Flow FFF retention data agree well with theoretical values.The retention increased and the peak shape became distorted at extreme pH conditions of the carrier solution.The selected carrier composition for comparison between the literature value of proteins was 0.05 M tris buffersolution with a pH of 8. Storing BSA at 4 ± 2