Humana Press eBooks · 2004 · 556 citations · 16 references
Life SciencesCell BiologyCell AdhesionCell PhysiologyWound HealingCell CultureCell MigrationCell TrackingExtracellular MatrixCell Migration AnalysisCell Migration AssaysCell Migration DynamicsCell Migration Through MatrixMigration Rate Estimation
The wound‑healing assay is a simple, inexpensive, early method to study directional cell migration in vitro, mimicking in vivo wound healing. The assay involves creating a wound in a cell monolayer, capturing images at the start and at regular intervals, and comparing them to quantify migration rate, with a variant that tracks individual cells at the leading edge. It is especially useful for studying how cell‑matrix and cell‑cell interactions affect cell migration.
The wound-healing assay is simple, inexpensive, and one of the earliest developed methods to study directional cell migration in vitro. This method mimics cell migration during wound healing in vivo. The basic steps involve creating a "wound" in a cell monolayer, capturing the images at the beginning and at regular intervals during cell migration to close the wound, and comparing the images to quantify the migration rate of the cells. It is particularly suitable for studies on the effects of cell-matrix and cell-cell interactions on cell migration. A variation of this method that tracks the migration of individual cells in the leading edge of the wound is also described in this chapter.
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Farin Kamangar, Graça M. Dores, William F. Anderson · Journal of Clinical Oncology · 2006 · 3.5K citations
Id1 and Id3 are required for neurogenesis, angiogenesis and vascularization of tumour xenografts
David Lyden, Alison Z. Young, David Zagzag et al. · Nature · 1999 · 934 citations
Medicine, Cancer Biology, Oncology +12