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An Orthogonal Biocatalytic Approach for the Safe Generation and Use of HCN in a Multistep Continuous Preparation of Chiral O-Acetylcyanohydrins

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2015

Year

Abstract

An enantioselective preparation of <i>O</i>-acetylcyanohydrins has been accomplished by a three-step telescoped continuous process. The modular components enabled an accurate control of two sequential biotransformations, safe handling of an <i>in situ</i> generated hazardous gas, and in-line stabilization of products. This method proved to be advantageous over the batch protocols in terms of reaction time (40 min <i>vs </i>345 min) and ease of operation, opening up access to reactions which have often been neglected due to safety concerns.