Concepedia
The EMBO Journal · 1996 · 1.3K citations · 74 references
Open access
Life SciencesBiochemistryMolecular BiologyCell SignalingSignal TransductionEndomembrane SystemProtein Kinase Cascades
74
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Kenneth J. Livak, Thomas D. Schmittgen · Methods · 2001 · 181.5K citations
Life Sciences, Molecular Biology, Molecular Genetics +9
A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
R Sikorski, P. Hieter · Genetics · 1989 · 8.8K citations · Full text
Life Sciences, Biotechnology, Genetics +17
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase
Donald B. Smith, Kevin S. Johnson · Gene · 1988 · 6K citations
Life Sciences, Biochemistry, Molecular Biology +12
[19] Rapid and efficient site-specific mutagenesis without phenotypic selection
Thomas A. Kunkel, John D. Roberts, Richard A. Zakour · Methods in enzymology on CD-ROM/Methods in enzymology · 1987 · 5.3K citations
Life Sciences, Biotechnology, Molecular Biology +8
XBP1 mRNA Is Induced by ATF6 and Spliced by IRE1 in Response to ER Stress to Produce a Highly Active Transcription Factor
Hiderou Yoshida, Toshie Matsui, Akira Yamamoto et al. · Cell · 2001 · 4.1K citations · Full text
Life Sciences, Cell Biology, Molecular Biology +13