The Effects of Different Levels of Salinity on Proline and A-, B- Chlorophylls in Canola

Abstract

In order to evaluation of response of canola to different levels of salinity an experiment was done in research laboratory of Islamic Azad University of Borujerd in Iran. Culturing seeds in the special dishes was done after sterilizing seeds by sodium hypochlorite solution 20%. Seedlings transferred to the Hogland- Arnon sub-power culture environment. After 24 hours from culturing, the seedlings were placed in the different salt medium (0, 75, 100 and 150 mM) as salinity treatments on Hayola and RGS. After 20 days the contents of a- and b- chlorophylls and proline were tested in the plant roots and leaves. Collected data analysis and mean comparison were done by SPSS software and Duncan's Multiple Range Test (DMRT). Results showed that salinity stress had significant effect on increase of a- and b- chlorophylls and content of proline (p<0.01). The amount of decreasing b-chlorophyll was more rather than a-chlorophyll. Decrease in roots and leaves was more in Hayola 401 cultivar than RGS cultivar. Salinity induced significant increase of proline content in leaf and root (p<0/01). Hayola cultivar had more ability to salinity stress tolerance compared with RGS.