Antioxidant Activity and HPTLC Analysis of Pandanus odoratissimus L. Root

Abstract

The methanolic and aqueous extracts of Pandanus odoratissimus L. root were examined for their contents of phenolics and flavonoids as well as for their in vitro antioxidant activities using 1, 1-diphenyl-2- picryl hydrazyl (DPPH) radical quenching assay and reducing power. The methanolicroot extract yielded 56.24 ± 0.02 mg/g GAE of phenolic content and 81.25 ± 0.046 mg/g CE of flavonoid content. Higher antioxidant potential was observed in both DPPH scavenging assay (EC = 48.3 ± 0.002 µg mL ) and reducing capacity 50 1 (OD at 1000 µg mL = 0.787) by the methanolic root extract than by the aqueous extract. A positive correlation 1 was found between phenolics and flavonoid contents and antioxidant properties of the extracts. These findings indicate that polyphenolic components may have antioxidant potential, as reported in other medicinal plants.