Response of Yield and Yield Components of Rice (Oryza sativa L.) to Pseudomonas flouresence and Azospirillum lipoferum under Different Nitrogen Levels

Abstract

In order to study the effect of Pseudomonas flouresence and Azospirillum lipoferum isolates on yield and yield components of rice (Oryza sativa L.) cultivar Tarom Daylamani, an experiment was conducted in Mazandaran province, city Neka in 1388. A split factorial experiment under complete randomized block design with three replications was used for this research. The first factor was pure bacterial isolates Azospirillum lipoferum (A) and Pseudomonas flouresence (B) and the combination of these two types of bacteria (A1B1) and the second factor included four levels of nitrogen (25, 50, 75, 100 kg ha) of urea. The analysis of variance showed that the effect of nitrogen fertilizer and bacteria had no significant difference on 1000 seeds weight, number of clusters (m2) and number of grains per panicle. The effect of fertilizers, bacteria Azospirillum lipoferum and Pseudomonas flouresence on rice yield showed that application of 100 kg of nitrogen with Pseudomonas and without Azospirillum had the highest yield of 5733 kg/ ha. Results of analysis of variance showed that fertilizers with bacteria, Pseudomonas flouresence and Azospirillum lipoferum had a significant effect on harvest index at P<0.01 level. Data also indicated that yield had the highest positive correlation with panicle and harvest index. The most important aim of this research was to reduce consumption of chemical fertilizers by using biological fertilizers which could lead to an effective step in preserving the environment.