Apoptogenic Activity of Secretion Extract of Bellamya Bengalensis f. Annandalei via Mitochondrial Mediated Caspase Cascade on Human Leukemic Cell Lines

Abstract

Secretion extract of Bellamya bengalensis f. annandalei have been traditionally used for many ailments. The anti-leukemic activity of secretion extract of Bellamya bengalensis f. annandalei (SEBB) has been investigated against three human leukemic cell lines U937, K562 and HL-60 where IC50 values are calculated to be 22.21µg/ml, 10.3µg/ml & 12.79µg/ml respectively. Morphologically, externalization of phosphatidyleserine has been found on all the treated cells but not in control cells. Cell shrinkage, membrane blebbing, chromatin condensation, nuclear fragmentation and formation of apoptotic bodies are characteristic features of apoptosis. Gel electrophoresis study shows fragmented DNA in the form of ladder and Flow cytometric analysis showed appreciable number of cells in early & late apoptotic stages. The cells are getting arrested in the sub-G1 & G1 phases of cell cycle. The apoptosis is mediated through activation of caspase-9 & Caspase-3.