High Levels of Foreign Gene Expression in Hepatocytes after Tail Vein Injections of Naked Plasmid DNA

TLDR

The liver technique requires direct injection into liver vessels (portal vein, hepatic vein, or bile duct) with outflow occlusion. Tail vein injections of naked plasmid DNA produce high levels of hepatocyte expression, especially when delivered rapidly in large volumes (~2.5 ml), and this method also works in muscle, offering broad laboratory utility.

Abstract

We have previously shown that the intramuscular injection of naked plasmid DNA enables foreign gene expression in muscle. Further studies showed that the intravascular delivery of naked plasmid DNA enables high levels of expression not only in muscle but also in hepatocytes. For the liver, this technique required injection directly into the liver vessels (portal vein, hepatic vein, or bile duct) and occlusion of outflow. The present study now demonstrates that high levels of plasmid DNA expression in hepatocytes can be easily obtained by tail vein injections. The highest levels of expression are achieved by rapidly injecting the plasmid DNA in large volumes, approximately 2.5 ml. This technique has great potential for a wide variety of laboratory studies.