Journal of Bacteriology · 2002 · 78 citations · 33 references
Life SciencesGeneticsMicrobiologyDna RepairGenetic EngineeringMicrobial GeneticsDna DamageDna Double-strand Break RepairDna Strand BreaksDouble-strand Break RepairHomologous RecombinationNucleotide Excision RepairDna Repair MechanismsGene DisruptionMicrobial Adaptation To Ultraviolet RadiationRepair Of Uv-induced Dna DamageTargeted Gene DisruptionUltraviolet-induced Dna DamageUv Stress ToleranceUv-induced Dna Lesions
An in vitro transposition system, developed to facilitate gene disruption in Deinococcus radiodurans R1, has been used to inactivate the gene designated dr1819 in uvrA-1(+) and uvrA-1 backgrounds. dr1819 encodes a protein with homology to a UV DNA damage endonuclease expressed by Schizosaccharomyces pombe. Interruption of dr1819 greatly sensitizes the uvrA-1 strain but not the uvrA-1(+) strain to UV light, indicating that the dr1819 gene product is a component in a DNA repair pathway that can compensate for the loss of nucleotide excision repair in this species. Clones of dr1819 will restore UV resistance to UVS78, a uvrA-1 uvsE strain, indicating that dr1819 and uvsE are the same locus.
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Genome Sequence of the Radioresistant Bacterium <i>Deinococcus radiodurans</i> R1
Owen White, Jonathan A. Eisen, John F. Heidelberg et al. · Science · 1999 · 967 citations