Family Clustering of Baeyer–Villiger Monooxygenases Based on Protein Sequence and Stereopreference

Abstract

The identification of enzyme pairs with overlapping substrate specificity and enantiocomplementary transformations is a key challenge in biocatalysis. Enantio- and regiodivergent Baeyer–Villiger oxidations were successfully carried out by using a small library of recombinant Escherichia coli strains expressing monooxygenases of various microbial origin (see picture). The clustering of enzymes based on stereopreference is in good agreement with phylogenetic similarity.