Selective Functionalization of the Protein N Terminus with N‐Myristoyl Transferase for Bioconjugation in Cell Lysate

Abstract

A site to behold: Robust site-specific functionalization of engineered proteins is achieved with N-myristoyl transferase (NMT) in bacterial cells. NMT tolerates non-natural substrate proteins as well as reactive fatty acid tags, rendering it a powerful tool for protein conjugation applications, including the construction of protein microarrays from lysate.