Enhancement and Modulation of Enzymatic Activity through Higher‐Order Structural Changes of Giant DNA–Protein Multibranch Conjugates

Abstract

A giant DNA leap for enzyme-kind: Giant DNA–protein multibranch conjugates were shown to be composed of a single β-lactamase enzyme (green, see scheme) conjugated to up to four 48.5 kbp lambda phage DNA branches (blue lines). The conjugation of giant DNA induces a two- to fourfold enhancement of the enzymatic activity, which is modulated by changes in the higher-order DNA structure. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.