Development of a Novel Screening Method for the Isolation of “ <i>Cronobacter</i> ” spp. ( <i>Enterobacter sakazakii</i> )

Abstract

A differential medium, "Cronobacter" screening broth, has been designed to complement agars based on hydrolysis of chromogenic alpha-glucopyranoside substrates. The broth was evaluated using 329 Enterobacteriaceae strains (229 target isolates), spiked/naturally contaminated samples, and a parallel comparison with current methods for raw materials, line/end products, and factory environment samples.