Concepedia

How to do successful gene expression analysis using real-time PCR

Stefaan Derveaux, Jo Vandesompele, Jan Hellemans

Methods · 2009 · 802 citations · 14 references

DOI

Concepts

References

14

A new mathematical model for relative quantification in real-time RT-PCR

Michael W. Pfaffl · Nucleic Acids Research · 2001 · 34.9K citations · Full text

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Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Jo Vandesompele, Katleen De Preter, Filip Pattyn et al. · Genome biology · 2002 · 20.1K citations · Full text

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The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

Stephen A. Bustin, Vladimı́r Beneš, Jeremy A. Garson et al. · Clinical Chemistry · 2009 · 16.1K citations · Full text

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qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data

Jan Hellemans, Geert Mortier, Anne De Paepe et al. · Genome biology · 2007 · 4.1K citations · Full text

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Quantification of mRNA using real-time RT-PCR

Tania Nolan, Rebecca Hands, Stephen A. Bustin · Nature Protocols · 2006 · 2.3K citations

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