Quantification of mRNA using real-time RT-PCR

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Jo Vandesompele, Katleen De Preter, Filip Pattyn et al. · Genome biology · 2002 · 20.1K citations · Full text

Life Sciences, Molecular Biology, Molecular Genetics +14

Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations

Michael W. Pfaffl, Aleš Tichopád, Christian Prgomet et al. · Biotechnology Letters · 2004 · 5.1K citations

Life Sciences, Genetics, Molecular Biology +12

Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data

Christian Ramakers, Jan M. Ruijter, Ronald H. Lekanne Deprez et al. · Neuroscience Letters · 2003 · 3.7K citations

Life Sciences, Biostatistics, Molecular Biology +9

The RIN: an RNA integrity number for assigning integrity values to RNA measurements

Andreas Schröeder, Odilo Mueller, Susanne Stocker et al. · BMC Molecular Biology · 2006 · 2.7K citations · Full text

Life Sciences, Molecular Biology, Computational Biology +11

IL-6 mediates hypoferremia of inflammation by inducing the synthesis of the iron regulatory hormone hepcidin

Elizabeta Nemeth, Seth Rivera, Victoria Gabayan et al. · Journal of Clinical Investigation · 2004 · 2.2K citations

Life Sciences, Immunology, Pathology +17