Direct Characterization of Enzyme–Substrate Complexes by Using Electrosonic Spray Ionization Mass Spectrometry

Abstract

It's a phase it's going through: Electrosonic spray ionization shows better desolvation efficiency than electrospray ionization and usually gives single-charge states when used to ionize noncovalent complexes of large biomolecules. The binary and ternary complexes of imidazole-3-glycerol phosphate synthase (IGPS) with its inhibitors appear to retain their native solution-phase conformations upon transfer into the gas phase (see picture).