A rapid HPLC-determination of ATP-related compounds and its application to herring stored under modified atmosphere

Abstract

Abstract A high performance liquid chromatography (HPLC) method was developed by using gradient elution with acetonitrile as a mobile phase to reduce the time required for determination of ATP, ADP, AMP, IMP, HxR and Hx. The method was then applied to investigate nucleotide degradation in herring kept in ice and MAP (60% CO2/40% N2).