Actin-Based Motility of <i>Burkholderia thailandensis</i> Requires a Central Acidic Domain of BimA That Recruits and Activates the Cellular Arp2/3 Complex

Abstract

Burkholderia species use BimA for intracellular actin-based motility. Uniquely, Burkholderia thailandensis BimA harbors a central and acidic (CA) domain. The CA domain was required for actin-based motility, binding to the cellular Arp2/3 complex, and Arp2/3-dependent polymerization of actin monomers. Our data reveal distinct strategies for actin-based motility among Burkholderia species.