Canadian Journal of Physiology and Pharmacology · 1973 · 22 citations · 0 references
PharmacyBiochemistryPharmacologyHepatic PharmacokineticsMetabolismSteroid BiochemistryBile Acid MetabolismBile FormationBiliary ExcretionBiliary Excretion Of MetabolitesHepatic ClearanceHepatic MetabolismBile Pigment ExcretionBile Pigment MetabolismBiliary Excretion KineticsBilirubin ClearanceBilirubin KineticsBilirubin MetabolismGlucuronidation PathwaysSteroid Glucuronidation
In rats, treatment with spironolactone or pregnenolone-16α-carbonitrile (PCN) accelerates bile flow and enhances hepatic microsomal bilirubin–UDP glucuronyltransferase activity (EC 2.4.1.17) and the biliary transport maximum (T m ) of bilirubin. The distribution of ethylanthranilate azo-pigments in fractions separated by thin-layer chromatography also indicates increased glucuronidation of bilirubin. These findings explain the mechanism of enhanced serum bilirubin disappearance in rats treated with the steroids.