Use of statistical tools in the study of the conditions of predation of<i>Duddingtonia flagrans</i>versus<i>Panagrellus</i>sp

Abstract

Abstract The objective of the present work was to study the conditions of predation of Duddingtonia flagrans conidia versus Panagrellus sp using response surface methodology. Conidia of D. flagrans (AC001) isolate were transferred into water-agar (WA) culture media at different pHs and different concentrations defined according to Central Composite Design (CCD). Five different concentrations of D. flagrans conidia: (1292, 500, 1000, 1500 and 1707) were used. For 2%WA media were used the following pHs were used: 6.29, 6.5, 7.0, 7.5 and 7.71. The response of the design corresponded to the number of larvae (transformed to square root scale) observed at the end of the experiment (10 days). At the tenth day, the non predated larvae were recovered in the Petri dishes. The results showed that 2%WA media at pH 7.0 contributed to improve the predatory activity of conidia of D. flagrans, and therefore this tool may be used in future studies under laboratory and natural conditions. Keywords: Nematophagous fungi Duddingtonia flagrans conidia Panagrellus spstatistical tools Acknowledgements The authors thank CNPq, FAPEMIG and CAPES for financial support. Additional informationNotes on contributorsJackson Victor Araújo Jackson Victor Araújo has obtained CNPq scholarship