Polish Journal of Environmental Studies · 2012 · 25 citations · 23 references
Life SciencesMicrobiologySoil SciencesMicrobial EcologyMicrobial GeneticsSoil MicrobiologyBacterial ClassificationBacterial IdentificationMicrobial DiversityMicrobial Population GeneticsPopulation Genetics Of MicrobesSoil Microbial GeneticsMolecular Identification Of AzotobacterPopulation Genetic DiversityRandom Amplified Polymorphic Dna AnalysisRandom Amplified Polymorphic Dna MarkersRestriction Fragment AnalysisSoil Bacterial DiversitySoil PropertiesStrain Isolation
The presence of Azotobacter sp. in soils has beneficial effects on plants, but the abundance of these bacteria is related to many factors, especially soil pH and fertility. The presented study evaluated the abundance of Azotobacter sp. in various soils of southern Poland and confirmed the relationship between soil properties and the presence of these bacteria. Diagnostic tests indicate that all isolates belonged to A. chroococcum species. The studied bacteria were present in 43% of soil samples. Restriction analysis of the bacterial Internal Transcribed Spacer region indicate that the selected isolates were identical. Two fingerprinting methods, PCR Melting Profile and Random Analysis of Polymorphic DNA, revealed high population diversity. Four (PCR MP) and five (RAPD) congruent clusters were defined. No correlation was found between the sources of strain isolation and their positions in clusters. PCR MP and RAPD techniques appeared to be useful in intraspecies differentiation of A. chroococcum.
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DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
John G. Williams, Anne R. Kubelik, Kenneth J. Livak et al. · Nucleic Acids Research · 1990 · 13.2K citations · Full text
Dhruva Bhattacharya, Priyangshu M. Sarma, Shibu Krishnan et al. · Applied and Environmental Microbiology · 2003 · 137 citations · Full text