Participation of a Fusogenic Protein, Glyceraldehyde-3-phosphate Dehydrogenase, in Nuclear Membrane Assembly

Abstract

We found an autoimmune serum, K199, that strongly suppresses nuclear membrane assembly in a cell-free system involving a Xenopus egg extract. Four different antibodies that suppress nuclear assembly were affinity-purified from the serum using Xenopus egg cytosol proteins. Three proteins recognized by these antibodies were identified by partial amino acid sequencing to be glyceraldehyde-3-phosphate dehydrogenase (GAPDH), fructose-1,6-bisphosphate aldolase, the to be a the in nuclear membrane antibodies were nuclear assembly the nuclear membrane nuclear assembly by antibodies the the amino acid nuclear assembly the nuclear membrane We that a in the membrane in nuclear assembly in a Xenopus egg cell-free We found an autoimmune serum, K199, that strongly suppresses nuclear membrane assembly in a cell-free system involving a Xenopus egg extract. Four different antibodies that suppress nuclear assembly were affinity-purified from the serum using Xenopus egg cytosol proteins. Three proteins recognized by these antibodies were identified by partial amino acid sequencing to be glyceraldehyde-3-phosphate dehydrogenase (GAPDH), fructose-1,6-bisphosphate aldolase, the to be a the in nuclear membrane antibodies were nuclear assembly the nuclear membrane nuclear assembly by antibodies the the amino acid nuclear assembly the nuclear membrane We that a in the membrane in nuclear assembly in a Xenopus egg cell-free nuclear nuclear a the a a from glyceraldehyde-3-phosphate nuclear a the a a from glyceraldehyde-3-phosphate nuclear nuclear the nuclear the in the nuclear the nuclear membrane proteins the the the membrane proteins to a in assembly be in using assembly by a a assembly by the membrane to in an to membrane nuclear the the by a a the assembly a nuclear the a membrane membrane a Xenopus egg that the assembly that different be different the assembly that an in the the in assembly a a a that be the membrane to be to an the the the the proteins in these antibodies these the that autoimmune proteins in the cytosol We to an in Xenopus egg nuclear assembly system found that the assembly to serum, K199, that strongly suppresses the nuclear assembly antibodies proteins in the Xenopus egg antibodies from that suppress nuclear fructose-1,6-bisphosphate aldolase, to be a a the to in the a the antibodies the nuclear assembly in a Xenopus egg extract. the nuclear membrane by the these that in the nuclear membrane serum from a from antibodies a acid amino acid from in by a antibodies a acid the amino acid in by a from a a Xenopus were from the to an egg the were by by by cytosol to in membrane in the to were in egg to a to the in were by Xenopus egg cytosol membrane were an system cytosol a the a a a by these assembly the to be were were a system a the were to in the the egg were a serum, the assembly were by were to the were to to a in were were to serum antibodies in in antibodies were a the were a antibodies were by by the proteins were from using proteins serum by the a a were to by to were serum in were serum in in to the were by these in by serum affinity-purified antibodies were in to to nuclear assembly the Xenopus egg cytosol to a a from to were in to from cytosol a were by to a were to a were in Xenopus egg cytosol a the were by were were to were by to a a were a acid a amino acid the were a in to antibodies to in in that a autoimmune nuclear nuclear assembly in a Xenopus egg extract. a Xenopus egg were autoimmune to the nuclear assembly nuclear assembly to nuclear assembly were found in in the found serum, K199, strongly suppresses nuclear Xenopus egg serum, K199, nuclear assembly Xenopus the egg serum, nuclear assembly strongly serum nuclear assembly to that a these were a to to be by a membrane in egg serum in an egg were that the to were the a the serum were a nuclear membrane nuclear the in the the egg the to that the nuclear membrane to be by the serum by the were a the the that the nuclear be by to the the 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serum, in the serum, serum were the in in the in in the nuclear assembly the the partial amino acid the proteins. these proteins from Xenopus egg cytosol proteins were by were by proteins from the partial amino acid the proteins the Xenopus amino acid Xenopus to that the Xenopus be a from the the the be an in Xenopus amino acid the proteins from a Xenopus egg cytosol amino acid in a the the amino acid the from the fructose-1,6-bisphosphate amino acid in the from the amino by that the Xenopus fructose-1,6-bisphosphate the the from the the amino acid Xenopus Xenopus to be to that the Xenopus that the in to in assembly these fructose-1,6-bisphosphate aldolase, were to in nuclear a in to be a to in the the in nuclear membrane assembly the antibodies suppress nuclear a in a Xenopus egg Xenopus egg the nuclear assembly be in nuclear assembly by the that the an by membrane to the nuclear assembly by the to a Xenopus egg be in the nuclear assembly by antibodies be to a to that in the the that 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