CONJUGATION OF SULFOBROMOPHTHALEIN SODIUM WITH GLUTATHIONE IN THIOETHER LINKAGE BY THE RAT *

Abstract

Most of the sulfobromophthalein sodium (BSP) appearing in the bile of man, rat, dog, sheep and cat differs chromatographically from the free BSP originally administered (1-4). The altered BSP compounds in man, rat and dog appear to be anmino acid conjugates Thus, after hydrolysis of the major BSP compounds with 6 N HC1, several amino acids have been detected by filter paper chromatography. Glycine, glutamic acid and ala- nine were identified in man (2, 3), glycine and glutamic acid in the rat (2) and glycine in the dog (3).