International Immunology · 2005 · 1.3K citations · 40 references
Life SciencesCell BiologyMolecular BiologyIntracellular TransportMembrane BiochemistryMembrane TraffickingExosome AnalysisVesicle TraffickingBiochemical CharacterizationExosomal Protein AnalysisExosome CharacterizationExosome Membrane CompositionExosome MorphologyExosome Surface MarkersMhc Class Ii MoleculesWestern Blot Analysis
Exosomes are small membrane vesicles (50–90 nm) secreted by most hematopoietic cells. The study reports the first evidence that exosomes circulate in human blood plasma. Exosomes were isolated from plasma by differential ultracentrifugation or CD63‑bead immunoisolation, then identified by electron microscopy and characterized biochemically via western blot and flow cytometry using tetraspanin antibodies. All 15 healthy donor plasma samples contained vesicles with exosome‑like size, density, and protein markers (CD63, CD9, CD81, MHC I/II, Lamp‑2) that floated at exosome densities, confirming that blood circulates exosomes and implying a role in intercellular communication.
Exosomes are small membrane vesicles (50-90 nm in diameter) secreted by most hematopoietic cells. We provide here the first evidence for the presence of exosomes in vivo, in the blood. Plasma samples of all healthy donors tested (n = 15) contain vesicles that are similar in shape, size and density to the previously described exosomes. They were clearly identified by electron microscopy after isolation by differential ultracentrifugation or immunoisolation with CD63-coated latex beads. We performed their biochemical characterization by western blot analysis and by flow cytometry after vesicle adsorption onto latex beads using a panel of mAbs. We observed that these plasma-derived vesicles contain tetraspanin molecules such as CD63, CD9, CD81 as well as class I and class II MHC molecules and Lamp-2 (i.e. proteins that are known to be enriched in exosomes). In addition, these vesicles float on sucrose gradient at a density similar to exosomes. Our results demonstrate that blood is a physiological fluid for exosome circulation in the body, suggesting their role in cell-cell or organ-organ communications as carriers for molecules that need to reach distant cell targets.
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B lymphocytes secrete antigen-presenting vesicles.
Graça Raposo, Hans W. Nijman, Willem Stoorvogel et al. · The Journal of Experimental Medicine · 1996 · 3.5K citations · Full text
Tumor-derived exosomes are a source of shared tumor rejection antigens for CTL cross-priming
Joseph Wolfers, Anne Lozier, Graça Raposo et al. · Nature Medicine · 2001 · 1.6K citations
Life Sciences, Immunology, Oncology +16