Characteristics of lipase isolated from coconut ( Cocos nucifera linn) seed under different nutrient treatments

Abstract

Lipase (triacyglycerol acyl hydrolase, E. C. 3.1.1.3) activity was demonstrated on acetone powder prepared from coconut seeds, under different nutrients condition. Conversion of the free fatty acid released to copper salt enabled the activity of the crude enzyme extract on coconut oil, triolein, tripalmitine and olive oil to be determined colorimetrically. Lipase from coconut plant grown under complete nutrient conditions showed high affinity for the native substrate coconut oil. The enzyme acted maximally at 35°C and had a broad optimum pH of 7.5 – 8.5.