Site specific protein immobilization into structured polymer brushes prepared by AFM lithography

Abstract

Protein immobilization into structured polymer brushes was achieved by AFM lithography. Dense polystyrene brushes with thicknesses larger than 40 nm show protein repellent properties whereas brushes with thicknesses smaller than 20 nm show the expected known protein adsorption behavior. Multi tip AFM lithography allows for large surface area patterning.