Publication | Closed Access
The Final Steps of Bacillaene Biosynthesis in <i>Bacillus amyloliquefaciens</i> FZB42: Direct Evidence for β,γ Dehydration by a <i>trans</i>‐Acyltransferase Polyketide Synthase
107
Citations
26
References
2010
Year
EngineeringMolecular BiologyAntibiotic BacillaeneBiosynthesisMetabolic EngineeringNatural Product BiosynthesisBiotransformationBiochemistryBiocatalysisFinal StepsMolecular MicrobiologyHplc–nmr SpectroscopyBacillaene BiosynthesisGenetic Pathway EngineeringBiomolecular EngineeringNatural SciencesBiotechnologyMicrobiologyγ DehydrationPathway Engineering
Caught in the act: Genetic pathway engineering in conjunction with HPLC–NMR spectroscopy of highly labile intermediates provided direct evidence for the installation of β,γ double bonds during polyketide elongation to form the antibiotic bacillaene. The novel congener bacillaene B (1) was also discovered as an elusive true end product of the pathway in Bacillus amyloliquefaciens.
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