Clinical and Experimental Pharmacology and Physiology · 2014 · 18 citations · 46 references
MedicineGeneticsMolecular GeneticsCardiovascular GeneticsGenetic EpidemiologyMedical GeneticsCardiovascular DiseaseCoronary Artery DiseaseDisease Susceptibility LociGenetic RiskGenetic SusceptibilityIschemic Heart DiseaseBinary Logistic RegressionCardiovascular Genetic RiskCoronary Heart Disease EpidemiologyDna ExtractionGenetic PolymorphismsLogistic RegressionMolecular Genetic DiagnosisTherapeutic Efficacy Assessment
The purpose of the present study was to clarify the association of eNOS 894G/T and ACE I/D genetic polymorphisms with the risk of coronary heart disease (CHD) and to explore the effects of these polymorphisms on the therapeutic efficacy of salvianolate injection in Chinese CHD patients. In all, 153 CHD patients and 198 healthy controls were enrolled in the study. We collected 5 mL peripheral blood for DNA extraction. Genetic diagnosis of eNOS 894G/T was determined by direct sequencing. Polymerase chain reaction-restriction fragment length polymorphism analysis was used to detect ACE I/D genotypes. We observed significant differences in the frequency distribution of eNOS and ACE polymorphisms between CHD patients and healthy controls (P < 0.05). Binary logistic regression stepwise analysis revealed that the genotypes had an additive and dominant effect on patients' therapeutic responses (P < 0.05). These data suggest that the genetic polymorphisms of ACE I/D and eNOS 894G/T probably play a role in the development of CHD and these genetic polymorphisms may affect the response to salvianolate injection in Chinese CHD patients.
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